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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Cancer Res. 2017 Aug 14;77(19):5313–5326. doi: 10.1158/0008-5472.CAN-17-0986

Figure 6. Loss of TP53 in ACHN cells rescued HNF1B loss induced cell death but not aneuploidy.

Figure 6

HNF1B or both HNF1B and TP53 were knocked down in ACHN cells. Cell proliferation was measured by either colony formation (A) or cell survival assay (B). HNF1B, p53, p21 and CDT-1 levels in ACHN cells were measured by immunoblotting (C). Chromosome number in mock, HNF1BKD and HNF1BKD+TP53KD cells was counted by metaphase spread assay. Images (D) and quantification results (E) are shown. Chromosome count distribution quantification results are shown in (F). 50 mitotic cells in each cell line were randomly counted. Data are expressed as mean±SD, N=3, *P<0.05; **P<0.01; ***P<0.001