Rb1	Forward 5′-AATGTGTTCCGTAAAAGTTTCTAGT-3′	Reverse 5′-TACACTTTCAGCTTTATGGGTTGG-3′
Mad1l1	Forward 5′-ATTTAGACTCACACTGCCGC-3′	Reverse 5′-AGGGATGCCCCTGAATGTCT-3′
Mad2l1	Forward 5′-GAGGGAATCCCCACCTCTTT-3′	Reverse 5′-AGCAAAACACAGAATGGCGAA-3′
Bub1b	Forward 5′-GCTTAGGCTACCTCCCTTTTT-3′	Reverse 5′-GAGACAGGGACTGGGAGAGA-3′
Rb1 −0.5kb	Forward 5′-TAAGATCTGTCCCGCTCGGA-3′	Reverse 5′-ACAAGGGAAGATGGGCGATG-3′
Rb1 0.7kb	Forward 5′-ATGCAACAGTTGGGAGCCTT-3′	Reverse 5′-AGGCTTTTCTTGGCCTCTCC-3′
Mad2l1 −0.5kb	Forward 5′-CTGCACAGTACGGACCTTGT-3′,	Reverse 5′-AATGAGCAAGCGCAGAGTG-3′
Mad2l1 0.5kb	Forward 5′-TCAGCGTGGCATTTATCCGT-3′,	Reverse 5′-ATGAGCTCGGGGTCAGTAGT-3′
Bub1b −0.5kb	Forward 5′-GGTGTTAAGACCTGGGGCTC-3′	Reverse 5′-TCTTCTGGGTTGCTCCGAAC-3′
Bub1b 0kb	Forward 5′-AATTTGAAACTTGGCGGCCC-3′	Reverse 5′-TCAACCTTCAGCTGGCTCTG-3′