SIN3B is required for PICS. A, Representative images of SA-β-Gal on fresh-frozen sections of the dorsolateral lobe of prostates taken from three-month-old Sin3bpc+/−
Ptenpc−/−, and Sin3bpc−/−
Ptenpc−/− mice. n = 2, 3 respectively. B, Representative images of immunohistochemistry for KI67, P21, pHP1γ, and IL6 on paraffin sections of the dorsolateral lobe of prostates taken from three-month-old Sin3bpc+/−, Sin3bpc−/−, Sin3bpc+/−
Ptenpc−/−, and Sin3bpc−/−
Ptenpc−/− mice. n = 3 each. Top Panel = 10× magnification. Bottom Panel = 40× magnification. Scale bar = 200 μm. C, Quantification of KI67 positive nuclei. n = 3, 3, 5, 5 respectively. Student’s t-test was used to assess significance. * p<0.05. D, P21. n = 3, 3, 4, 5 respectively. E, pHP1γ. n = 3, 3, 5, 6 respectively.