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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Alcohol Clin Exp Res. 2017 Sep 14;41(10):1705–1714. doi: 10.1111/acer.13473

Figure 5.

Figure 5

In vitro acetylation of rSOD2 by acetic anhydride to determine susceptibility of each lysine residue toward acetylation and enzyme inhibition. (A) Western blotting using anti-acetyl-lysine antibody demonstrates an increase in SOD2 acetylation due to non-enzymatic acetylation and Coomassie blue staining shows protein abundance. (B) Acetylated rSOD2 was examined via LC-MS/MS analysis to identify acetylated lysine residues at each concentration of acetic anhydride. (C) SOD2 activity was inhibited as a result of lysine acetylation (P< 0.01). (Mean ± SEM, n=3).