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. 2017 Oct 3;7:12580. doi: 10.1038/s41598-017-12996-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ATR-AP205-001 was drained to popliteal lymph node and deposited in follicle dendritic cell (FDC) area. Mice popliteal lymph nodes were acquired at 15 mins, 2 hrs, 8 hrs, 24 hrs and 4 days after footpad injection. (A) Distribution of ATR-AP205-001 (upper, green) and ATR-Dimer-001 (lower, green) in popliteal lymph node. Red arrows figure out the accumulation of antigens inside the lymph node. Scale bars, 200 μm. (B) Proportion of cells participating in antigen-capturing. (C) Co-localization of ATR-AP205-001 (upper, green) and ATR-Dimer-001 (lower, green) with IgD⁺ follicle B cells (blue) and CD21/35⁺ FDCs (red). The antigens deposited in FDC area were annotated by white arrow. Scale bars, 100 μm. n = 6 per group. Data are expressed as mean ± SEM.