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. 2017 Oct 3;7:12580. doi: 10.1038/s41598-017-12996-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tfh cells enhancement after ATR-AP205-001 vaccination. The expression of co-stimulatory molecules CD80 (A) and CD86 (B) on DCs at 0 min, 2 hrs, 10 hrs, 1 day, 2 days and 4 days post vaccination (n = 6 per group). (C) PD-1^highCXCR5^high Tfh cells, gated on CD4⁺ T cells. (D) Proportion of Tfh cells in dLNs and spleen on days 7 and 14 (n = 7–9 per group). (E) Relative mRNA expression of Bcl-6 and IL21 in dLNs and spleen on day 14 (n = 7–9 per group). Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.