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. 2017 Oct 3;7:12580. doi: 10.1038/s41598-017-12996-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ATR-AP205-001 promoted anti-ATR001 antibody production and long-lasting B cell memory. (A) ATR001-specific antibody secreting cells on days 14 and 21. (B) IgG isotypes of ATR-AP205-001 and ATR-Dimer-001. (n = 6 per group). (C) ATR001-specific memory B cells gated as ATR001⁺ CD19⁺ IgD⁻ IgG⁺ CD38⁺. (D) Proportion of ATR001-specific B cells. (E) Memory B cells in dLNs and spleen on the 150th days after second immunization (n = 7 per group). Data are presented as mean ± SEM. *P < 0.05 vs. control group.