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. 2017 Oct 3;7:12622. doi: 10.1038/s41598-017-13025-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of relative erlotinib concentrations among necrotic, viable tumor, and normal lung regions in the mouse xenograft model (A) Erlotinib tissue distribution by matrix-assisted laser desorption/ionization mass spectrometry imaging and regions of interest are shown. (B) Relative erlotinib concentrations were estimated from the mass spectrometry images and liquid chromatography-tandem mass spectrometry using serial sections, and were compared among necrotic, viable tumor, and normal mouse lung regions in the mouse xenograft model. Error bar = standard deviation of replicate analysis for each region of interest.