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. 2017 Oct 3;7:12620. doi: 10.1038/s41598-017-13093-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IAA delay soybean seed coat rupture and radicle protrusion. The imbibition seeds treated by IAA (1 μM) and H₂O were investigated by stereomicroscope (Leica M165 C, Germany) with different time points (0, 3, 6, 9, 12, 15, 18 hour after sowing). (A) The representative photographs of seed coat rupture of IAA and H₂O treatment soybean seeds during imbibition. (B) Length of radicle of germinated soybean seeds 48 hours after IAA treatment. (C) Fresh weight of germinated soybean seeds 48 hours after IAA treatment. Bar in panel A = 50 mm. The average percentages of four repeats ± standard error are shown. **Difference is significant at the 0.01 level.