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. 2017 Oct 3;7:12638. doi: 10.1038/s41598-017-12988-y

Figure 6.

Figure 6

Tissue-specific expression analysis of grape HB genes. Seedless grape cultivar ‘Thompson Seedless’ is denoted as ‘T.S’ and seeded grape cultivar ‘Red Globe’ is denoted as ‘R.G’. (a) Semi-quantitative RT-PCR analysis. For each gene, yellow and blue color scale indicates high and low expression levels respectively. Transcripts were normalized to the expression of the ACTIN1 gene and EF1-α gene, and results are shown in Supplementary Figs S3 and S5. Semi-quantitative RT-PCR was analyzed using GeneTools software, and expression values were normalized based on the mean expression value of each gene in all tissues. The heat map was analyzed using MeV 4.8 software. (b) Real-time PCR validation of twelve genes (9 differentially expressed genes and 3 randomly selected genes) expressed in different tissues. Transcripts were normalized to the expression of the ACTIN1 gene; the mean ±SD of three biological replicates is presented.