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. 2017 Oct 3;7:12638. doi: 10.1038/s41598-017-12988-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression profiles of grape HB genes during ovule development in four grape cultivars. Two seedless grape cultivars, ‘Thompson Seedless’ and ‘Flame Seedless’, are denoted as ‘T.S’ and ‘F’ respectively. Two seeded grape cultivars, ‘Red Globe’ and ‘Kyoho’, are denoted as ‘R.G’ and ‘K’ respectively. (a) Semi-quantitative RT-PCR analysis. For each gene, yellow and blue color scale indicates high and low expression levels, respectively. Numbers indicate the number of days after full bloom (DAF). Transcripts were normalized to the expression of the ACTIN1 gene and EF1-α gene and the results are shown in Supplementary Figs S4 and S5. Semi-quantitative RT-PCR measurements were analyzed using GeneTools software, and data was normalized based on the mean expression value of each gene in all development stages. The heat map was analyzed using MeV 4.8 software. (b) Real-time PCR validation of twelve genes (9 differentially expressed genes and 3 randomly selected genes) expressed during ovule development in four grape cultivars. Transcripts were normalized to the expression of the ACTIN1 gene; the mean ± SD of three biological replicates is presented.