Table 1.
Primer sequences and the expected PCR product sizes.
| Name | Note | Sequences (5′ to 3′) | Product size |
|---|---|---|---|
| P1773-w385A | FUT2 wild-type forward | AGGAGGAATACCGCCACAT | 575 bp |
| P1774-m385T | FUT2 mutated forward | GAGGAGGAATACCGCCACT | 576 bp |
| P1775-w428G | FUT2 wild-type forward | GCTACCCCTGCTCCTGG | 529 bp |
| P1776-m428A | FUT2 mutated forward | CGGCTACCCCTGCTCCTA | 530 bp |
| P1777-w571C | FUT2 wild-type forward | TAGGGGTC CATGTTCGCC | 389 bp |
| P1778-m571T | FUT2 mutated forward | GTAGGGGTCCATGTTCGCT | 390 bp |
| P1772-fut2 rev | FUT2 reverse primer | GGCTGCCTCTGGCTTAAAG | / |
| P1779-hGH | hGH forward | GCCTTCCCAACCATTCCCTT | 428 bp |
| P1780-hGH rev | hGH reverse | TCACGGATTTCTGTTGTGTTTC | / |