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. 2017 Oct 3;7:12584. doi: 10.1038/s41598-017-12936-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression in pituitary and testis from GCF and WT males. Expression of fshb (a), lhb (b), gnrhr4 (c), amh (d), igf3 (e), insl3 (f), star (g), cyp17a1 (h), cyp11β (i), fshr (j), and lhcgr (k) relative to ef1a, in Atlantic salmon male pituitaries (a–c) and testes (d–k), measured by qPCR. Data are shown as mean with SEM, n = 21–22 (GCF), 8–10 (Immature WT) and 8–9 (Mature WT). Significant differences between groups are indicated by different letters. GCF; germ cell-free, WT; wild type.