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. 2017 Oct 3;7:12593. doi: 10.1038/s41598-017-12827-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of folding and unfolding rate constants calculated in the absence of denaturant of PDZ3 (empty circles) and PDZ3Δα3 (filled circles) at different experimental conditions. The rate constants were obtained by analysis of the chevron plots reported in Fig. 3. The folding rate constant k _F is very similar for both PDZ3 and PDZ3Δα3 at all the experimental conditions explored. Data recorded at different pH conditions, left column, where fitted to the Henderson–Hasselbalch equation, returning a single transition with a robust apparent pK _a of about 3.5. In all cases, an increase in the microscopic unfolding rate constant could be observed, consistent with a destabilization of the native state. The overall dependence of the unfolding rate constants were however unaffected by the truncation of α3.