Figure 1.

TAK1 deletion leads to increased expression of NUMBL and decreased expression of TRAF6 and NEMO, which gets rescued by exogenous expression of WT-TAK1. Bone marrow cells were isolated from WT or TAK1-cKO mice and cultured in presence of M-CSF. (A) Bone marrow cells were treated with or without RANKL and TNFα for 3 days in the presence of M-CSF. Western blots shows Increased NUMBL expression along with decreased TRAF6 and NEMO expression in TAK1-cKO cells when compared with WT cells. (B) WB showing decreased TRAF6, NEMO and TAK1 expression in TAK1-cKO cells compared to WT bone marrow cells, after short-term RANKL treatments. (C) Bone marrow cells were isolated from WT or TAK1-cKO mice were transfected with pMX-GFP, pMX-TAK1 and pMX-K63W and cultured in presence of M-CSF for 3 days followed by western blotting. WB showing rescue of TRAF6 and NEMO expression in TAK1-cKO cells expressing TAK1-WT but not in TAK1-cKO cells expressing catalytically inactive TAK1-K63W. Full-length Western blots images are included in supplementary data as Figure S1.A-C.