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. 2017 Oct 3;7:12600. doi: 10.1038/s41598-017-12707-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Deletion of NUMBL in myeloid lineage leads to increase in osteoclastogenesis. Bone marrow cells were isolated from WT and NUMBL-cKO mice. (A) Cells were cultured in 96 well plate in the presence of RANKL and M-CSF for 4 day followed by fixation, TRAP staining and (B) counting. Bone marrow cells from WT and NUMBL-cKO mice were treated with RANKL for 0, 2 and 4 days in the presence of M-CSF, followed by RNA isolation for qPCR of OC differentiation associated genes. NUMBL deletion results in increased expression of OC differentiation associated genes (C) TRAP, (D) NFATc1, (E) DC-STAMP, (F) β3-Integrin, (G) Cathepsin-K and (H) MMP9 in bone marrow cells upon RANK stimulation. (**P < 0.01 and ***P < 0.001).