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. 2017 Oct 3;7:12599. doi: 10.1038/s41598-017-12787-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Expression pattern analysis of bovine SIX1 in tissues and organs. SIX1 mRNA expression was normalized against that of the housekeeping gene β-actin and expressed relative to gene expression in the liver. The value of each column represents the mean ± standard deviation based on three independent experiments. Unpaired Student’s t-test was used to detect significant differences. “*”P < 0.05 and “**”P < 0.01. (b) Bovine SIX1 expression pattern at different tissues and organs. (c) Phylogenetic tree analysis of SIX1. We calculated 8000 bootstrap replicates to bootstrap confidence values.