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. 2017 Oct 3;7:12599. doi: 10.1038/s41598-017-12787-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Structural characteristics of the bovine SIX1 gene. (a) The detailed genomic, mRNA and protein components containing the 5′/3′-untranslated region (5′/3′-UTR), and the open reading frame (ORF). (b) 5′ Regulatory region sequence of the bovine SIX1 gene. Arrows mark the transcription initiation sites. The translational start site (ATG) is shown in red letters. The transcription factor binding sites are boxed, and primer sequences are underlined with the respective names shown below the line. The CpG island is indicated by a blue box. (c) Schematic representation of the relative loci of the E-box motif, MyoD, CEBP, SIX4, CREB, PAX7, MyoD, MEF2, TEAD4 and MEF3 motif binding sites in the SIX1 promoter. Arrows mark the motifs and transcription factors recognition sites. Dashed lines indicate the GC percentage as represented on the y-axis and the x-axis denotes the bp position in the 5′ untranslated region. Coordinates are given relative to the translational start site (shown as +1).