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. 2017 Oct 3;7:12599. doi: 10.1038/s41598-017-12787-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EMSAs showing direct binding of MyoD, PAX7, CREB and MyoG to the SIX1 promoter in vitro. Nuclear protein extracts were incubated with 5′-biotin labelled probe containing the MyoD, PAX7, CREB or MyoG binding site in the presence or absence of competitor (lane 2), 50× mutation probe (lane 3) and 50× unlabelled probes (lane 4). The super-shift assay was conducted using 10 μg of anti-MyoD, anti-PAX7, anti-CREB or anti-MyoG antibodies (lane 5). The arrows mark the main complexes. Muscle NE, QCMC nuclear protein extracts (a,b,c and d). Adipocytes NE, adipocyte nuclear protein extracts (e).