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. 2017 Oct 3;7:12644. doi: 10.1038/s41598-017-13011-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative images of fluorescently labelled tracer poliovirus to demonstrate homogenous composition of the 3 IPV types coated onto the Nanopatch. The coating solution contained 1% methyl cellulose, 0.75% trehalose, 0.75% sucrose, 0.35% mannitol and (a) IPV1 with dylight 647 shown in blue, (b) IPV2 with dylight 550 shown in red, (c) IPV3 with dylight 488 shown in green. (d) merge of images a-c with areas of complete overlap shown as white. Images representative of n = 4 replicates.