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. 2017 Oct 3;7:12615. doi: 10.1038/s41598-017-12667-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Summary of genes affected by expression of RpoN* in P. aeruginosa PAO1. Expression of RpoN* interferes with the ability of RNA polymerase and native RpoN to bind to sig54 promoters altering the transcriptional profile of P. aeruginosa PAO1. Summaries of areas of gene expression that are specifically altered by RpoN* are shown and include the genes encoding the transcriptional regulators RhlR, Anr, GbdR, and their respective regulons. These results are consistent with RpoN binding sites identified by ChIP-seq.²⁴. Expression of RpoN* was shown to alter expression of a number of other genes including hypothetical (~250) and ribosomal (~70) genes. A full list of these genes is available in Supplementary Table S2.