Table 5.
Comparison of relaxation dispersion parameters derived from 3D 15N relaxation dispersion experiment on Abp1p SH3 domain partially bound to Ark1p peptide using co-MDD and CS-VE methods at 8.3, 7.0, 5.8 and 4.2% NUS levels
| Parameter | 15N CPMG | |||||||
|---|---|---|---|---|---|---|---|---|
| co-MDD | IRLS-VE | |||||||
| 8.3% | 7.0% | 5.8% | 4.1% | 8.3% | 7.0% | 5.8% | 4.1% | |
| Exchange rate (s−1) | 211 ± 30 | 206 ± 20 | 206 ± 23 | 209 ± 28 | 246 ± 27 | 235 ± 30 | 243 ± 35 | 274 ± 54 |
| Population of the excited state (%) | 2.8 ± 0.3 | 2.9 ± 0.2 | 2.9 ± 0.3 | 2.8 ± 0.3 | 2.5 ± 0.2 | 2.6 ± 0.3 | 2.6 ± 0.3 | 2.5 ± 0.4 |
| RMSD between |Δϖcpmg| and |Δϖdirect|(ppm) | 0.22 | 0.22 | 0.24 | 0.24 | 0.26 | 0.25 | 0.26 | 0.3 |
Except for 8.3% NUS, reported values and errors correspond to mean and standard deviation of the related parameters respectively over 20 resampling trials. R2 errors at all NUS levels were estimated via jackknife resampling