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. 2017 Jul 28;36(5):425–432. doi: 10.1007/s10930-017-9734-x

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Expression of Nudt13 in High Five™ cells. High Five™ cells were infected with recombinant Nudt13 virus at a MOI of 10 for 48 h. Samples were analysed by SDS-PAGE (15% w/v) and stained with Coomassie Blue. a Lane 1 protein standards: bovine serum albumin (66 kDa), ovalbumin (45 kDa), glyceraldehyde 3-phosphate dehydrogenase (36 kDa), carbonic anhydrase (29 kDa), trypsinogen (24 kDa), soybean trypsin inhibitor (20 kDa) and α-lactalbumin (14.2 kDa); lane 2 control uninfected High Five™ cells; lane 3 high Five™ cells infected with Nudt13 virus for 48 h; lane 4 purified Nudt13. b Immunoblot analysis of Nudt13 (the same cells as in a, lane 3) using His.Tag monoclonal antibody