Table 1.
Substrate specificity of Nudt13 was determined colorimetrically at a fixed substrate concentration of 0.5 mM
| Substrate | Relative activity (%) | Substrate | Relative activity (%) |
|---|---|---|---|
| NADH | 100 | Ap4A | 2 |
| NADPH | 92 | Ap6A | 0.5 |
| NAD+ | 7 | UDP-glucose | 2 |
| NADP+ | 4 | UDP-galactose | 0.5 |
| FAD | 7 | Canonical NTPs | <0.1 |
| ADP-ribose | 5 | Canonical dNTPs | <0.1 |
| Ap2A | 14 | 8-oxo-dGTP | <0.1 |
| Ap3A | 4 | 5-Me-CTP | <0.1 |
The activity was expressed relative to NADH hydrolysis under the same conditions, where 100% was 5.7 µmol NADH hydrolyzed min−1 mg−1 protein. Values are the averages of duplicate determinations