Fig. 1.

AHR antagonists increase the yield of in vitro differentiated pDC. Cord blood CD34⁺ progenitors were cultured for 2 weeks in the absence (CTL—control) or in the presence of AHR antagonists (CH223191 or StemRegenin-1, SR1). Absolute numbers of differentiated pDC were determined by using immunostaining and flow cytometry analysis. a Differentiated pDC were identified as HLA-DR⁺CD123^hi by flow cytometry. Representative contour plots are presented with percentage of pDC for each culture condition. b Absolute numbers of pDC obtained from 10⁵ CD34⁺ cells are presented with median (n = 7 independent experiments). Statistical analyses were performed by using one-way analysis of variance. **p < 0.01, ***p < 0.001