Fig. 6.
Activated ivD-pDC infusions cure human ALL in humanized mice. Irradiated newborn NSG mice were transplanted with 104 human cord blood-derived CD34+ cells. 105 REH ALL cells were intravenously injected in humanized mice 8 weeks after transplantation. Forty-eight hours after leukemia injection, mice were injected with activated pDC (a-pDC; n = 12) or unstimulated pDC (unst-pDC, n = 5) (once a week, for 5 weeks), 20,000 IU of IL-2 (daily injections; n = 7) or saline solution for control mice (control; n = 18). a In vivo bioluminescence imaging of ALL-bearing mice was performed weekly. Images of representative mice are shown; units in rainbow color scales are proportional to the numbers of photons per second. b Survival curves of ALL-bearing humanized mice treated with unstimulated or TLR9-activated ivD-pDC, IL-2 or saline solution injections. Mice were euthanized after overt leukemia onset. Flow cytometry analysis of bone marrow samples confirmed complete leukemia involvement. Log-rank test was used to compare survival