Table 1.
Summary of sample demographic and health information and analyses performed
| ID | Agea | Sexb | Smoker | Oral health | Sampled tooth | Analyses performed |
|---|---|---|---|---|---|---|
| Modern | ||||||
| OK1010 | 40 | M | Former | + PD | − Caries, + PD | UPLC–MS/MS |
| ES28 | 31 | F | Current | + Caries, + GV | − Caries, − PD | UPLC–MS/MS |
| ES29 | 30 | M | No | + Caries, + GV | − Caries, − PD | UPLC–MS/MS |
| ES20 | 23 | M | Current | + Caries, + GV | − Caries, − PD | UPLC–MS/MS |
| ES15 | 71 | M | Former | + Caries, + PD | − Caries, + PD | UPLC–MS/MS |
| Historic | ||||||
| CS06 | 36–45 | M | N/A | + Caries, + PD | − Caries, − PD | UPLC-MS/ MSc; GC-MS |
| CS12 | 36–45 | M | N/A | + Caries, + PD | − Caries, − PD | UPLC–MS/MSc; GC–MS |
| CS18 | 36–45 | M | N/A | − Caries, + PD | − Caries, − PD | UPLC–MS/MSc; GC–MS |
| CS24 | 36–45 | F | N/A | + Caries, + PD | + Caries, + PD | UPLC–MS/MSc; GC–MS |
| CS46 | > 45 | F | N/A | + Caries, + PD | − Caries, − PD | UPLC–MS/MSc; GC–MS |
| CS20 | 36–45 | M | N/A | + Caries, + PD | − Caries, − PD | UPLC–MS/MS |
| CS21 | 26–35 | M | N/A | − Caries, − PD | − Caries, − PD | UPLC–MS/MS |
| CS23 | 26–35 | F | N/A | + Caries, + PD | − Caries, − PD | UPLC–MS/MS |
| CS30 | 18–25 | F | N/A | + Caries, − PD | − Caries, − PD | UPLC–MS/MS |
| CS31 | 36–45 | F | N/A | + caries, + PD | − Caries, − PD | UPLC–MS/MS |
| CS39 | 36–45 | M | N/A | − Caries, + PD | − Caries, − PD | UPLC–MS/MS |
| CS40 | > 45 | F | N/A | − Caries, − PD | − Caries, − PD | UPLC–MS/MS |
PD periodontal disease, GV gingivitis, N/A not available, + present, − absent
aAge of skeletons used for historical calculus sampling is based on osteological indicators and is a range estimate
bHistorical skeleton sex estimate is based on both osteological indicators and DNA sequencing, and was in concurrence between the two methods
cUPLC–MS/MS was performed twice; the first at Metabolon, Inc. and the second at the University of Wisconson-Madison