Fig. 1.

Production of cytokines by monocyte-derived dendritic cells matured in the presence of different stimulatory cocktails. Monocytes from two donors were screened for the production of IL-12p70 after an initial 48 h culture with GM-CSF/IL-4 followed by harvest, wash, and thereafter 18 h with GM-CSF/IL-4 together with different combinations of TNFα, IFNγ, R848, and the GMP-grade poly I:C Hiltonol^® (a). Monocytes from three donors were matured into mDC with the same stimulators as above and also LPS. The supernatants were screened for the production of a panel of different cytokines (b). Numbers represent concentrations (pg/ml) of the respective cytokine and the gray scale represents the lowest (white) to highest (black) concentrations within each donor. Stars indicate that the measured cytokine was added to some cocktails (~35,000 pg/ml IFNγ or 20,000 pg/ml TNFα). Monocytes from three donors were screened for the production of IL-12p70 after an initial 48 h culture with GM-CSF/IL-4 followed by 18 h with IFNγ, R848, Hiltonol, and LPS or with the “gold standard” (TNFα, IL1β, IL-6, PGE2) and “alpha type-1” (TNFα, IL-1β, IFNα, IFNγ, Hiltonol) DC cocktail (c)