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. 2017 Jun 10;66(10):1333–1344. doi: 10.1007/s00262-017-2029-4

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Expression of maturation markers by monocyte-derived dendritic cells matured with different stimulatory cocktails. Monocytes from three donors were cultured for 48 h with GM-CSF/IL-4 followed by harvest, wash, and thereafter 18 h with GM-CSF/IL-4 together with different combinations of TNFα, IFNγ, R848, the GMP-grade poly I:C Hiltonol^®, and LPS. Thereafter the surface expression of CD80 (a), CD83 (b), CD86 (c), HLA-DR (d), CCR7 (e), IL-15Rα (f), surface-bound IL-15 (g), PDL1 (h), was assessed by flow cytometry. Depicted values are mean fluorescent intensities