Table 1.

LPS-induced transcripts most affected by BSO in Groups 1 and 2.

2 h				6 h
GeneSymbol	Genbank accession	P-value	log2FC	GeneSymbol	Genbank accession	P-value	log2FC
Most up-regulated by BSO (Group 1)
Ptpn14a	NM_008976	<0.0001	1.85	Hunk	NM_015755	<0.0001	1.97
Serpinb1ba	NM_173052	<0.0001	1.60	Serpinb1ba	NM_173052	0.0002	1.85
Ccdc54a	NM_027046	0.0100	1.31	Gclma	NM_008129	<0.0001	1.80
Srxn1a	NM_029688	<0.0001	1.28	Hunk	NM_015755	<0.0001	1.73
Tex19.1a	NM_028602	<0.0001	1.22	Ptpn14a	NM_008976	<0.0001	1.52
Hunka	NM_015755	0.0007	1.14	Serpina3g	NM_009251	0.0017	1.42
Esda	NM_016903	0.0002	1.14	Slc39a4a	NM_028064	<0.0001	1.40
Procr	NM_011171	<0.0001	1.13	Mep1aa	NM_008585	0.0032	1.21
Tfeca	NM_031198	0.0046	1.03	Tfeca	NM_031198	0.0026	1.14
Slc7a11a	NM_011990	<0.0001	1.00	Prdx1	NM_011034	<0.0001	1.12
3110068a07rika	AK039947	0.0033	0.97	Fosl1	NM_010235	0.0082	1.11
Prdx1a	NM_011034	<0.0001	0.97	Gema	NM_010276	0.0275	1.09
Hunka	NM_015755	0.0008	0.94	Slc7a11a	NM_011990	<0.0001	1.07
Zdhhc18	NM_001017968	<0.0001	0.70	Acta2a	NM_007392	<0.0001	1.01
Zfpm2	NM_011766	0.0006	0.69	Vwce	NM_027913	0.0009	1.00
Most down-regulated by BSO (Group 2)
Sox14	NM_011440	0.0093	−1.52	Nup62cl	NM_001081668	<0.0001	−4.34
Olfr1412	NM_146277	0.0005	−1.51	Psg26a	NM_001029893	<0.0001	−4.21
il1b	NM_008361	0.0004	−1.49	Kif12a	NM_010616	0.0052	−2.56
Fabp2	NM_007980	0.0093	−1.48	LOC102634429	XM_006521612	0.0244	−2.29
Mx2	NM_013606	0.0329	−1.39	C230030N03Rik	AK082264	0.0034	−2.22
Otor	NM_020595	0.0017	−1.29	Pdia2	NM_001081070	0.0068	−1.79
Zfp811	NM_183177	0.0021	−1.13	Adrbk2	AK048763	0.0125	−1.65
Edn1	NM_010104	0.0003	−1.04	Glipr1l1	NM_027018	0.0052	−1.56
Il4i1	NM_010215	0.0003	−1.04	Urah	NM_029821	0.0103	−1.36
Oas2	NM_145227	<0.0001	−1.03	Urah	NM_029821	0.0168	−1.25
5830411N06Rik	AK030813	0.0022	−1.00	Cabp4	NM_144532	0.0129	−1.20
Zfp46	NM_009557	0.0031	−0.99	Tcp10c	NM_001167578	0.0050	−1.18
Irf7	NM_016850	0.0383	−0.92	ND6	AK140300	<0.0001	−1.13
Saa3	NM_011315	0.0015	−0.89	Adora2a	NM_009630	0.0021	−1.07
Pdgfb	NM_011057	0.0002	−0.86	Faaha	NM_010173	0.0023	−0.94

Transcripts were selected for their differential expression between the two groups BSO + LPS vs LPS alone (cutoff was: FC, 1.5; P < 0.05 by one-way ANOVA, followed by a correction for multiple comparisons by controlling the false discovery rate with the two-stage step-up method of Benjamini, Krieger, and Yekutieli). Data for both 2 and 6 h are reported. Only the top 15 transcripts most affected by BSO (sorted by FC) are shown.

^aTranscripts that are also significantly affected by BSO alone (BSO vs control, with a cutoff of FC 1.5, P < 0.05 as above). The full list can be seen in File S1 in Supplementary Material. Transcripts in bold were selected for PCR validation.

ANOVA, analysis of variance; BSO, buthionine sulfoximine: FC, fold change; LPS, lipopolysaccharide.