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. 2017 Sep 29;8:1734. doi: 10.3389/fmicb.2017.01734

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Copyright © 2017 Dulebohn, Richards, Su, Lawrence and Gherardini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lactate and acetate are encountered by B. burgdorferi during the infectious cycle. Representative HPLC chromatograms monitoring absorbance at 210 nm of samples prepared from (A) mouse sera, (B) rabbit sera, (C) B. burgdorferi-infected tick midgut material, or (D) a standard containing lactate, acetate, propionate, and butyrate. Arrows indicate peaks confirmed by mass spectroscopy corresponding to lactate, acetate, propionate, and butyrate with their relative retention times listed in minutes. Asterisk indicates that the sample was diluted 1:1 with water prior to being run on the HPLC. Benzoate has a retention time of 30.1 min and is not displayed on this chromatogram. (E) Mean concentration of lactate or acetate found in mouse sera, rabbit sera, or the B. burgdorferi-infected tick midgut determined by HPLC analysis. Quantification of lactate and acetate was performed in biological triplicate for mouse sera and tick midgut contents. One rabbit blood sample was taken and sample preparation and HPLC/MS analysis was performed in technical triplicate.