Skip to main content
. 2017 Sep 29;8:1242. doi: 10.3389/fimmu.2017.01242

Figure 2.

Figure 2

Low-dose BLM induces fibrotic alterations in the skin of WT and S1P5−/− mice. (A) Representative skin sections of mice treated 4 weeks with BLM or PBS stained histochemically with Masson’s trichrome to indicate fibrotic alterations (scale bar = 100 µm, blue = connective tissue). (B) Dermal thickness and (C) expansion of connective tissue of WT and S1P5−/− mice treated 2 and 4 weeks with BLM were determined in microscopic images of stained skin sections (see Materials and Methods). BLM-treated WT mice are represented as black bars, and S1P5−/− mice are represented as gray bars. All results are shown as mean ± SD of n = 4–5 mice/group with # indicating p ≤ 0.05, ## for p ≤ 0.01 and ### for p ≤ 0.001, compared with the respective PBS controls (adjacent white bars). Statistical analysis was performed using a one-way ANOVA with Bonferroni’s multiple comparison test. ANOVA, analysis of variance; BLM, bleomycin; PBS, phosphate-buffered saline; WT, wild type.