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. 2017 Sep 29;8:1847. doi: 10.3389/fmicb.2017.01847

Table 3.

Characterization of ParRS-dependent MexXY overproducing mutants among the 14 strains exhibiting low oprD mRNA levels.

Strains Gene expressiona ParRS primary sequence
mexY PA1797 ParR ParS
24362 72.57 7.50 wtb R185Gc
25333 16.77 95.35 wtb A215Tc
25334 0.73 nd nd nd
25356 33.05 35.02 wtb A215Tc
25752 1.09 nd nd nd
26103 3.40 1.10 wtb wtc
26427 40.17 11.60 wtb R185Gc
26860 1.74 nd nd nd
26877 9.44 47.60 wtb A138Tc
30124 21.71 15.40 wtb A324Vc
35941 23.37 5.70 L11Fb wtc
36150 2.74 nd nd wtc
37249 1.18 nd nd wtc
37257 49.76 23.90 wtb L50Pc
a

Relative to expression in strain PAO1. Values are means from two independent experiments, each including duplicate determinations. Genes mexY and PA1797 are considered as significantly up-regulated (in boldface) when their respective expression is at least four-fold higher than those in PAO1.

b

PAO1-like sequence.

c

PA14- and LESB58-like sequence.

nd, not determined; wt, wild-type sequence.