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Purification and activity of recombinant FcrA. (A) SDS-PAGE of 8 μg His6-tagged FcrA purified from RHA1. (B) GC/MS trace of a reaction mixture containing 1.4 μM FcrA-His6, 100 μM oleoyl-CoA, and 400 μM NADPH (20 mM Tris-HCl, pH 7.0, 50 mM NaCl) incubated for 20 h.
