TABLE 3.
Primers used for PCR detection of resistance genes
| Gene | Forward oligonucleotide sequence (5′ to 3′) | Reverse oligonucleotide sequence (5′ to 3′) | Expected size (bp) | Reference |
|---|---|---|---|---|
| blaCMY-2 | GACAGCCTCTTTCTCCACA | TGGAACGAAGGCTACGTA | 1015 | 76 |
| blaPSE-1 | TTTGGTTCCGCGCTATCTG | TACTCCGAGCACCAAATCCG | 150 | 77 |
| blaTEM | GCACGAGTGGGTTACATCGA | GGTCCTCCGATCGTTGTCAG | 860 | 78 |
| aadA | GTGGATGGCGGCCTGAAGCC | AATGCCCAGTCGGCAGCG | 528 | 79 |
| aadA2 | CGGTGACCATCGAAATTTCG | CTATAGCGCGGAGCGTCTCGC | 250 | 80 |
| strA | CCTGGTGATAACGGCAATTC | CCAATCGCAGATAGAAGGC | 548 | 79 |
| strB | ATCGTCAAGGGATTGAAACC | GGATCGTAGAACATATTGGC | 509 | 79 |
| sul1 | CGGACGCGAGGCCTGTATC | GGGTGCGGACGTAGTCAGC | 591 | 75 |
| sul2 | GCGCTCAAGGCAGATGGCATT | GCGTTTGATACCGGCACCCGT | 285 | 78 |
| cmlA | TGGACCGCTATCGGACCG | CGCAAGACACTTGGGCTGC | 641 | 75 |
| tet(A) | GCTACATCCTGCTTGCCTTC | CATAGATCGCCGTGAAGAGG | 210 | 40 |
| tet(B) | TTGGTTAGGGGCAAGTTTTG | GTAATGGGCCAATAACACCG | 659 | 40 |
| tet(C) | CTTGAGAGCCTTCAACCCAG | ATGGTCGTCATCTACCTGCC | 418 | 40 |
| tet(G) | CAGCTTTCGGATTCTTACGG | GATTGGTGAGGCTCGTTAGC | 844 | 40 |