TABLE 3.
Activities toward 0.5 mM α-naphthyl estersa
| Enzyme | Sp act (U/mg) |
|||
|---|---|---|---|---|
| C2 (acetate) | C3 (propionate) | C4 (butyrate) | C8 (octanoate) | |
| Wild type | 21.9 ± 1.7 | 9.3 ± 0.3 | NDb | ND |
| Y39G mutant | 2.7 ± 0.2 | 0.87 ± 0.06 | ND | 0.03 ± 0.01 |
| Y39P mutant | 5.1 ± 0.2 | 1.5 ± 0.02 | ND | ND |
| Y39A mutant | 5.1 ± 0.3 | 1.6 ± 0.3 | ND | ND |
| W160P mutant | 1.1 ± 0.4 | 1.7 ± 0.2 | 0.31 ± 0.09 | ND |
| W160S mutant | 1.0 ± 0.1 | 1.5 ± 0.1 | 0.85 ± 0.09 | 0.05 ± 0.04 |
| W160A mutant | 1.1 ± 0.1 | 1.6 ± 0.1 | 0.84 ± 0.17 | 0.07 ± 0.06 |
| Y39G/W160P mutant | 1.8 ± 0.1 | 0.88 ± 0.06 | 0.59 ± 0.09 | ND |
| Y39P/W160S mutant | 0.49 ± 0.02 | 0.59 ± 0.06 | 0.04 ± 0.01 | 0.02 ± 0.01 |
| AlFAEAc | 1.3 | 1.4 | 3.1 | 0.070 |
a
Activities were measured in 85 mM potassium phosphate buffer (pH 7.0) at 37°C. Activity for α-naphthyl decanoate (C10) was not detected for all enzymes.
b
ND, not detected.
c
Data from Koseki et al. (39).