TABLE 4.

Activities toward methyl esters of 1 mM methyl hydroxycinnamates^a

Enzyme	Sp act (U/mg)
	MpCA	MCA	MFA	MSA
Wild-type	ND	ND	ND	ND
Y39G mutant	ND	ND	ND	ND
Y39P mutant	ND	ND	ND	ND
Y39A mutant	ND	ND	ND	ND
W160P mutant	6.9 × 10−4 ± 0.5 × 10−4	1.8 × 10−4 ± 0.1 × 10−4	1.7 × 10−3± 0.1 × 10−3	1.2 × 10−4 ± 0.1 × 10−4
W160S mutant	1.1 × 10−3 ± 0.1 × 10−3	1.6 × 10−3 ± 0.03 × 10−3	5.7 × 10−3 ± 0.2 × 10−3	3.7 × 10−4 ± 0.2 × 10−4
W160A mutant	1.8 × 10−3 ± 0.2 × 10−3	5.5 × 10−4 ± 0.1 × 10−4	3.9 × 10−3 ± 0.2 × 10−3	1.7 × 10−4± 0.1 × 10−4
Y39G/W160P mutant	4.7 × 10−3 ± 0.4 × 10−3	1.4 × 10−2 ± 0.1 × 10−2	2.0 × 10−2 ± 0.1 × 10−2	2.3 × 10−3 ± 0.4 × 10−3
Y39P/W160S mutant	3.3 × 10−3 ± 0.6 × 10−3	8.7 × 10−3± 0.2 × 10−3	1.5 × 10−2 ± 0.04 × 10−2	1.5 × 10−3 ± 0.1 × 10−3
NcFAE1b	20.9	8.2	8.97	ND
PfFAEBc	36.1	31.7	22.9	ND

^aActivities were measured in 95 mM potassium phosphate buffer (pH 7.0) at 37°C. ND, not detected.

^bData from Crepin et al. (23).

^cData from Kroon et al. (22).