TABLE 5.

Kinetic parameters toward MFA^d

Enzyme	kcat (s−1)	Km (mM)	kcat/Km (s−1 mM−1)
W160P mutanta		>10	4.2 × 10−4 ± 0.3 × 10−4
W160S mutanta		>10	1.4 × 10−3 ± 0.5 × 10−3
W160A mutant	8.4 × 10−3 ± 1.1 × 10−3	8.3 ± 0.2	1.0 × 10−3 ± 0.4 × 10−3
Y39G/W160P mutant	3.2 × 10−2 ± 0.5 × 10−2	8.0 ± 2.0	4.0 × 10−3 ± 0.2 × 10−3
Y39P/W160S mutant	3.1 × 10−2 ± 0.3 × 10−2	6.6 ± 1.2	4.8 × 10−3 ± 0.1 × 10−3
NcFAE1b	5.2	0.25	21
PfFAEBc	16	0.047	340

^aThe K_m and k_cat values could not be confidently determined due to the solubility limits of the substrate (10 mM).

^bData from Crepin et al. (23).

^cData from Kroon et al. (22).

^dActivities were measured in 95 mM potassium phosphate buffer (pH 7.0) at 37°C.