TABLE 6.
Ferulic acid-releasing activities toward 1% feruloylated insoluble wheat arabinoxylanc
| Enzyme | Sp act (mU/mg)a |
|
|---|---|---|
| After 24 h | After addition of xylanase (1 h)b | |
| Wild type | ND | ND |
| Y39G mutant | ND | ND |
| Y39P mutant | ND | ND |
| Y39A mutant | ND | ND |
| W160P mutant | 0.019 ± 0.001 | 0.20 ± 0.01 |
| W160S mutant | 0.055 ± 0.01 | 0.94 ± 0.46 |
| W160A mutant | 0.029 ± 0.01 | 0.44 ± 0.01 |
| Y39G/W160P mutant | 0.010 ± 0.007 | 0.060 ± 0.02 |
| Y39P/W160S mutant | 0.12 ± 0.02 | 1.5 ± 0.2 |
| AlFAEA | 7.5 ± 0.57 | 319 ± 21 |
a
Calculated from released ferulic acid after indicated time of incubation. ND, not detected.
b
Xylanase from Thermomyces lanuginosus (10 μg/ml) was added.
c
Activities were measured in 50 mM potassium phosphate buffer (pH 7.0) at 37°C.