Figure 1.
Soluble trehalase (A), Membrane-bound trehalase activity (B), and trehalose (C), and glucose (D) levels in N. lugens at different developmental stages from 0 h in 5th instar nymph to 72 h in adults. All of N. lugens 5th instar nymph and adults were selected every 12 h, and insects were collected and used to detect the activities of the two kinds of trehalase isoenzymes and measure the trehalose and glucose levels. Every group had three to five replicates. The age of the brown planthoppers was defined as follows: 5L-0, 0 h fifth-instar nymph; 5L-12, 12 h fifth-instar nymph; 5L-24, 24 h fifth-instar nymph; 5L-36, 36 h fifth-instar nymph; 5L-48, 48 h fifth-instar nymph; 5L-60, 60 h fifth-instar nymph; 5L-72, 72 h fifth-instar nymph; 5L-84, 84 h fifth-instar nymph; A-0, 0 h adults; A-12, 12 h adults; A-24, 24 h adults; A-36, 36 h adults; A-48, 48 h adults; A-60, 60 h adults; and A-72, 72 h adults. (Tukey's test, α = 0.05, A>B>C>D).