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Expression of NlTRE1-1, NlTRE1-2, and NlTRE2 in the five different tissues of N. lugens analyzed. Total RNA was extracted from head, leg, wing bud, cuticle, and fat body tissues, and the expression of both genes was measured by quantitative real-time PCR using 18S RNA as the internal control. Values are the means ± standard errors (SEs) from three independent measurements. The relative expression of each gene was determined in relation to that obtained in the head of N. lugens adults.
