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. 2017 Sep 26;8:750. doi: 10.3389/fphys.2017.00750

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Copyright © 2017 Zhang, Qiu, Yang, Wang, Zhou, Wang and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in the mRNA transcript levels in wing bud and abnormal phenotypes of the three NlTRE genes 48 h after injection with specific RNAi constructs and validamycin in N. lugens nymph. (A–C) Represent the mRNA levels of NlTRE1-1, NlTRE1-2, and NlTRE2 after the injection of RNAi targeting the NlTRE genes or validamycin normalized relative to the Nl-18S mRNA level. (D) Normal and different deformity phenotypes and wing curling. Last-instar nymphs were chosen as the targets for dsRNA injection. ^*indicated significant differences at P < 0.05, and ^**Indicated significant differences at P < 0.01.