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Effects of different dsTRE or validamycin solutions on the relative expression levels of chitin synthase in wing bud tissue. And CHS1 (A), CHS1a (B), and CHS1b (C) genes' expression level at 48 h normalized relative to the NL-18S mRNA levels, as measured by qRT-PCR. *Indicated significant differences at P < 0.05, and **Indicated significant differences at P < 0.01.
