Table 3.
Primers used for qRT-PCR measurements of chitin metabolism genes.
| Gene | GenBank number | Forward (5′–3′) | Reverse (5′–3′) | Length of target fragment (bp) |
|---|---|---|---|---|
| NlCHS1 | AEL88648 | CCGCAAACGATTCCTACAGA | AGGTCCTTGACGCTCATTCC | 222 |
| NlCHS1a | JQ040014 | TGTTCTTGCTACAACTCAATAAA | ACACCAATCCGATAGGCTC | 141 |
| NlCHSb | JQ040013 | GCTGTCTTTGCTTTCTTCAT | ACACCAATCCGATAGGCTC | 187 |
| NlCht1 | AJO25036 | AGGTGGTTAGGGACGAGGAG | TGCGCTTGACATAGTTGGACT | 114 |
| NlCht2 | AJO25037 | GCAGATTTCTGGACAGGGAA | TGACGCACAAGCGGGAAG | 226 |
| NlCht3 | AJO25038 | CTACACCTCTGGCTAAACTCGG | AACTTGTCCTTGCGGCTGAT | 235 |
| NlCht4 | AJO25039 | TTGAGGAGGTTCACGGGTCT | CCTTACTGGAAACGAGGTTGG | 112 |
| NlCht5 | AJO25040 | AAAGCGTTCGTGATGAAATAGC | GATCCTTTGCCTCAATCCAAT | 183 |
| NlCht6 | AJO25041 | GCTGGTAAGGAGATGCTATTCG | GTGGTTCTAAGGCTGGCTGTC | 155 |
| NlCht7 | AJO25042 | CTACTCTGCCATCCCATTCCT | GTCTGGGTTTCTTCACTTCCTG | 161 |
| NlCht8 | AJO25043 | GAACAAAGTGCAAACTCAGTC | C CACCTTCTGTGGCTTCTGG | 106 |
| NlCht9 | AJO25044 | GTGCGGTATTGGTTGAAGAGG | GGTATAACGTGATTCCGAGCC | 147 |
| NlCht10 | AJO25045 | CAAGCCAATACCCAACAAAC | ACAGCAAATCCATAGAGCACA | 177 |
| IDGF | AJO25056 | AAAAGAACGAGGAGGAGGG | TTGCTTGAGGATGGGGTAC | 170 |
| ENGase | AJO25057 | TGTGGCAAGACTTCGTTA | ATGGGAGGGTTGGGATAG | 282 |