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. 2017 Sep 25;8:667. doi: 10.3389/fphar.2017.00667

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Copyright © 2017 Bosma, Witt, Vaas, Josimovic, Gribbon, Vischer, Gul and Leurs.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histamine-induced intracellular calcium mobilization in HeLa cells endogenously expressing the H₁R. (A) Calcium dependent fluorescence was measured over time after injection of increasing histamine concentrations at t = 0. Representative graphs are shown of ≥3 experiments depicting the mean ± SD of duplicate values (n = 2). (B) For comparison, peak response and area under the curve (AUC) were determined from the kinetic calcium mobilization traces and plotted against the histamine concentration.