Figure 5.

High-dose cisplatin cross-preserves hair cells exposed to toxic doses of gentamicin. (A) Mouse organ of Corti explant cultures exposed to 100 μM gentamicin (Gent) for 24 h results in significant hair cell loss (top panels). Additional supplementation of 500 μM cisplatin to the toxic concentration of gentamicin rescues the hair cells, albeit with severely reduced MYO7A immunoreactivity (bottom panels). (B) Outer hair cell counts per 100 μm (basal turn) after exposure to gentamicin with or without high dose cisplatin (Unpaired t-test, p-value: < 0.0001, n = 6 organs). (C) Gentamicin uptake into cochlear cells was visualized by immunocytochemistry using a gentamicin-specific antibody. There was no difference in gentamicin immunoreactivity within the cochlear hair cells between gentamicin alone or with high dose cisplatin. Scale bar 20 μm.