Figure 3.
Decreased pyoverdine production by pvdS and NPRS mutants. The four pvdS mutants (PA_D19, PA_D21, PA_D22 and PA_D24) and PA_D25 produced significantly less pyoverdine compared with their ancestor strains, which do not carry any mutations in genes involved in pyoverdine synthesis pathway. Complementation of PA_D19, PA_D21 and PA_D22 with pUCP18::pvdS restored their pyoverdine production to the similar levels as their ancestors. We could not complement the PA_D24 strain with pUCP18::pvdS due to its high resistance to carbenicillin.