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. 2017 Sep 13;7(9):170076. doi: 10.1098/rsob.170076

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© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — Purification of histones from asynchronous and nocodazole arrested cells. (a) A scheme for purification of histones from DT40 cells while preserving cell-cycle-regulated post-translational modifications. (b) A typical Coomassie-stained SDS-PAGE gel of purification steps: Lane 1—acid lysis supernatant. Lanes 2–6—dilution series of pooled ion-exchange eluate. Lanes 7–12—elution fractions after size exclusion chromatography (SEC). (c) Summary of mass spectrometry (MS) analysis of the isolated histones. Plot of log₁₀ of peptide intensities and log₂ of mitotic enrichment. Red, histones; blue, ribosomal proteins; grey, other contaminating proteins. Inset pie chart shows the total sum of peptide intensities for these proteins.