Figure 4.

Chromatin reconstituted with histones derived from mitotic cells aggregates even in the absence of divalent cations. (a) DNA was absorbed onto poly-l-lysine-coated glass and stained with Hoechst dye. (b) Reconstituted interphase chromatin absorbed onto poly-l-lysine-coated glass was stained with Hoechst dye. In all panels, low (i) and high (ii) exposures are shown together with a blow-up of the high exposure (iii). (c) Reconstituted interphase chromatin treated with MgCl₂ was absorbed onto poly-l-lysine-coated glass and stained with Hoechst dye. (d) Reconstituted mitotic chromatin was absorbed onto poly-l-lysine-coated glass and stained with Hoechst dye. (e) Reconstituted mitotic chromatin treated with MgCl₂ absorbed onto poly-l-lysine-coated glass and stained with Hoechst dye. Scale bar, 5 µm.