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A partial multiple sequence alignment of ophiuroid TRH-1 precursors showing clade-specific gain/loss of neuropeptide copies. Mono- and dibasic cleavage sites are highlighted in green, mature peptides in red with the glycine residue for amidation in pink. Species have been grouped and coloured (clade A in purple, clade B in blue and clade C in orange) based on the phylogeny determined by O'Hara et al. [12].
